FITC Annexin V 640905 from BioLegend

Product Specs
ItemFITC Annexin V
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640905
Conjugate/TagFITC
ApplicationsFlow Cytometry (FC)
Quantity25 tests
Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Nameannexin A5
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
TargetAnnexin V
SpeciesHuman, Mouse, Rat

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Graft conditioning with fluticasone propionate reduces graft‐versus‐host disease upon allogeneic hematopoietic cell transplantation in miceEMBO Molecular MedicineSeptember 11, 2023Erika S Varady, L Angel Ayala, Pauline U Nguyen, Vanessa M Scarfone, Alborz Karimzadeh, Cuiwen Zhou, Xiyu Chen, Scott A Greilach, Craig M Walsh, Matthew A Inlayusing the dead cell stain propidium iodide (PI) and the apoptotic cell marker Annexin V (Biolegend, #s 640905 and 640911) according to the manufacturer's protocol. For transplantation, each well containing splenocytes

(2) Phosphatidylserine-Exposing Annexin A1-Positive Extracellular Vesicles: Potential Cancer BiomarkersVaccinesMarch 13, 2023Perez GI, Bernard MP, Vocelle D, Zarea AA, Saleh NA, Gagea MA, Schneider D, Bauzon M, Hermiston T, Kanada M.mixed with 0.2% BSA, APC-labeled anti-CD63 antibodies (A15712, Invitrogen), 0.8 µg/mL FITC-annexin A5 (640905, BioLegend) or 4.8 µg/mL FITC-GlaS in annexin binding buffer [10 mM HEPES (pH 7.4), 140 mM NaCl, 2.5

(3) Pluripotency retention and exogenous mRNA introduction in planarian stem cells in cultureiScienceFebruary 17, 2023Kai Lei, Wenya Zhang, Jiajia Chen, Sean A. McKinney, Eric J. Ross, Heng-Chi Lee, Alejandro Sánchez AlvaradoCY-SC007 CellTracker Green CMFDA Dye,ThermoFisher,Cat# C7025 Annexin V FITC Conjugate,BioLegend,Cat# 640905 Dextran-FITC,ThermoFisher,Cat# D3306 DAPI,ThermoFisher,Cat# D3571 mMESSAGE mMACHINE T7 ultra kit,ThermoFisher,Cat#

(4) Generating in vitro models of NTRK-fusion mesenchymal neoplasia as tools for investigating kinase oncogenic activation and response to targeted therapyOncogenesisFebruary 17, 2023Fabio Vanoli, Laurie Herviou, Yusuke Tsuda, Patricia Sung, Ziyu Xie, Eve Fishinevich, Soe S. Min, William Mallen, Henry de Traux de Wardin, Yanming Zhang, et al.were collected after treatment with TRK inhibitors and labeled using FITC Annexin V kit (Biolegend, 640905) according to manufacturer’s instruction and propidium iodide (PI), followed by flow cytometry and quantification

(5) Immunotherapy reverses glioma-driven dysfunction of immune system homeostasisJournal for ImmunoTherapy of CancerFebruary 7, 2023Bayli DiVita Dean, Tyler Wildes, Joseph Dean, Oleg Yegorov, Changlin Yang, David Shin, Connor Francis, John W Figg, Mathew Sebastian, Laura Falceto Font, et al.with extracellular fluorescent flow cytometry antibodies, then stained with Annexin V (Biolegend cat. 640905) in Annexin binding buffer (BD cat. 556454). Both forward/side scatter discrimination and gating on

(6) Impaired CXCL12 signaling contributes to resistance of pancreatic cancer subpopulations to T cell-mediated cytotoxicityOncoImmunologyDecember 31, 2022Yuan-Na Lin, Marcel O. Schmidt, Ghada M. Sharif, Eveline E. Vietsch, Amber J. Kiliti, Megan E. Barefoot, Anna T. Riegel, Anton Wellstein

(7) IRF4 deficiency vulnerates B-cell progeny for leukemogenesis via somatically acquired Jak3 mutations conferring IL-7 hypersensitivityCell death and differentiationNovember 1, 2022Das Gupta D, Paul C, Samel N, Bieringer M, Staudenraus D, Marini F, Raifer H, Menke L, Hansal L, Camara B, et al.routinely above 95%. To determine cell viability, AnnexinV/PI staining was performed using 5 µL AnnexinV (640905, Biolegend) per 500 µL HBSS. After 20 min of incubation at room temperature in the dark, 1 µL PI (421301,

(8) METTL14-dependent m(6)A modification controls iNKT cell development and functionCell reportsAugust 2, 2022Cao L, Morgun E, Genardi S, Visvabharathy L, Cui Y, Huang H, Wang CR.facility,N/A N6-methyladenosine 5′-monophosphate sodium,MilliporeSigma,Cat#: M2780–10MG FITC Annexin V,BioLegend,Cat#: 640905 7AAD,BD Biosciences,Cat#: 559925 Recombinant Murine IL-15,Peprotech,Cat#: 210–15 Recombinant murine IL-2

(9) Downregulation and Hypermethylation of GABPB1 Is Associated with Aggressive Thyroid Cancer FeaturesCancers (Basel)March 8, 2022Xiangling Xing, Ninni Mu, Xiaotian Yuan, Na Wang, C. Christofer Juhlin, Klas Strååt, Catharina Larsson, Shi Yong Neo, Dawei Xuproliferation. 2.9. Flow Cytometry For apoptosis assays, FITC Annexin V (Biolegend, San Diego, CA, USA, 640905) was used to stain cells, and fluorescence signals were determined. To detect markers of epithelial-to-mesenchymal

(10) Dysregulated lipid metabolism blunts the sensitivity of cancer cells to EZH2 inhibitorEBioMedicineMarch 1, 2022Zhang T, Guo Z, Huo X, Gong Y, Li C, Huang J, Wang Y, Feng H, Ma X, Jiang C, et al.

(11) TAT–RHIM: a more complex issue than expectedBiochemical JournalFebruary 11, 2022Benedikt Kolbrink, Theresa Riebeling, Nikolas K. Teiwes, Claudia Steinem, Hubert Kalbacher, Ulrich Kunzendorf, Stefan Krautwald

(12) Nicotinamide riboside kinase-2 inhibits JNK pathway and limits dilated cardiomyopathy in mice with chronic pressure overloadClinical science (London, England : 1979)January 28, 2022Shahzadi SK, Marzook H, Qaisar R, Ahmad F.

(13) Cationic Polymer Brush-Modified Carbon Nanotube-Meditated eRNA LINC02569 Silencing Attenuates Nucleus Pulposus Degeneration by Blocking NF-κB Signaling Pathway and Alleviate Cell SenescenceFrontiers in Cell and Developmental BiologyJanuary 17, 2022Yulin Huang, Jiaming Yang, Xizhe Liu, Xiaoshuai Wang, Kai Zhu, Zemin Ling, Baozhu Zeng, Ningning Chen, Shaoyu Liu, Fuxin Wei

(14) Nemo-Dependent, ATM-Mediated Signals from RAG DNA Breaks at Igk Feedback Inhibit V κ Recombination to Enforce Igκ Allelic ExclusionThe Journal of ImmunologyJanuary 15, 2022Rebecca A. Glynn, Craig H. BassingAPC Rat anti-mouse IgM (BD, 550676, Clone 11/41), and then stained with FITC Annexin V (BioLegend, 640905) in Annexin V Binding buffer (10mM HEPES, 140mM NaCl, and 35mM CaCl2) and then propidium iodide solution

(15) Photodynamic therapy of melanoma with new, structurally similar, NIR-absorbing ruthenium (II) complexes promotes tumor growth control via distinct hallmarks of immunogenic cell deathAmerican journal of cancer researchJanuary 1, 2022Konda P, Roque Iii JA, Lifshits LM, Alcos A, Azzam E, Shi G, Cameron CG, McFarland SA, Gujar S.

(16) Systemically Silencing Long Non-coding RNAs Maclpil With Short Interfering RNA Nanoparticles Alleviates Experimental Ischemic Stroke by Promoting Macrophage Apoptosis and Anti-inflammatory ActivationFrontiers in cardiovascular medicineJanuary 1, 2022Wang Y, Liu C, Chen Y, Chen T, Han T, Xue L, Xu B.instruction, and siRNA usage was 1 mg/kg. Apoptosis Assay Cells were stained using FITC Annexin V (Cat# 640905, Biolegend), counterstained with propidium iodide (Cat# P8080, Solarbio, China), and analyzed on the

(17) Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cellsNucleic acids researchDecember 16, 2021Jackson LM, Dhoonmoon A, Hale A, Dennis KA, Schleicher EM, Nicolae CM, Moldovan GL.

(18) SLPI is a critical mediator that controls PTH-induced bone formationNature CommunicationsDecember 1, 2021Akito Morimoto et al.(1:100, 405203, BioLegend), and Streptavidin-FITC (1:50, 405201, BioLegend), Annexin V-FITC (1:50, 640905, BioLegend), and 7AAD (559525, BD Pharmingen, San Diego, CA, USA). RNA-Seq analysis For in vivo osteoblast

(19) Roxadustat Does Not Affect Platelet Production, Activation, and Thrombosis FormationArteriosclerosis, Thrombosis, and Vascular BiologyOctober 1, 2021Jiaxin Zhao, Yanyan Xu, Jingyuan Xie, Junling Liu, Ruiyan Zhang, Xiaoxiang Yanconcentrations of thrombin in the presence of fluorescein isothiocyanate–annexin V (50 μL/mL; catalog No. 640905; BioLegend) at 37 °C for 40 minutes. Samples were further diluted with Tyrode buffer, and the proportion

(20) Phosphatidylserine binding directly regulates TIM-3 functionThe Biochemical journalSeptember 17, 2021Smith CM, Li A, Krishnamurthy N, Lemmon MA.flow cytometry PerCP-CyTM5.5 or FITC-labeled annexin V were obtained from BioLegend (catalog #640936 and 640905). As specified by the manufacturer, cells were washed twice with ice-cold PBS and then resuspended in

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FITC Annexin V from BioLegend

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FITC Annexin V from BioLegend

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